European Commission
EU Wildlife Trade Regulations

Types of biological samples referred to in Article 18 of Regulation (EC) No 865/2006 and their use1

This Annex lists the types of biological samples for which certain procedures which are less strict may apply.

Type of sample Typical size of sample Use of sample
blood and its derivative components 5 ml maximum for liquid samples or dry blood sample on a microscope slide, filter paper or swab biomedical research; species identification; determination of geographic origin; sex determination; individual identification; parentage testing; toxicology analysis; disease testing/diagnosis, including serology
internal tissues (botanical or zoological), fixed tissues (5 mm3-25 mm3) in a fixative or histological glass slide containing a +/-5um section of fixed tissue Histology and electron microscopy to detect organisms and poisons; taxonomic research; biomedical research; species identification; determination of geographic origin; sex determination; individual identification; parentage testing; toxicology analysis; disease testing/diagnosis
internal tissues (botanical or zoological), frozen pieces of tissues (5 mm3-25 mm3) biomedical research; species identification; determination of geographic origin; sex determination; individual identification; parentage testing; toxicology analysis; disease testing/diagnosis
internal tissues (botanical or zoological), fresh (excluding ova, sperm and embryos) pieces of tissues (5 mm3-25 mm3) biomedical research; species identification; determination of geographic origin; sex determination; individual identification; parentage testing; toxicology analysis; disease testing/diagnosis
external tissues including hair, skin, feathers, scales, bone, egg shell, teeth, ivory, horn, leaves, bark, seeds, fruit or flowers Individual samples with or without fixative.
For ivory: pieces of ivory approximately 3 cm x 3 cm and 1 cm thick or less depending on analysis method, in accordance with ICCWC Guidelines on methods and procedures for ivory and laboratory analysis2.
For rhino horn: small amounts of powder/shavings sealed in a tamper proof sample bottle, in accordance with the Procedure for Rhino horn DNA Sampling3.
species identification; determination of geographic origin; sex determination; individual identification; parentage testing; toxicology analysis; disease testing/diagnosis; age analysis; biomedical research
buccal/cloacal/mucus/nasal/urinary tract/rectal swabs small amounts of tissue or cells on a swab in a tube species identification; determination of geographic origin; sex determination; individual identification; parentage testing; toxicology analysis; disease testing/diagnosis, including serology; biomedical research
cell lines and tissue cultures no limitation of sample size biomedical research; species identification; determination of geographic origin; sex determination; individual identification; parentage testing; toxicology analysis; disease testing/diagnosis; age analysis
DNA or RNA (purified) up to 0.5 ml volumes per individual specimen of purified DNA or RNA biomedical research; species identification; determination of geographic origin; sex determination; individual identification; parentage testing; toxicology analysis; disease testing/diagnosis; age analysis
secretions (saliva, venom, milk, plant secretions) 1-5 ml in vials production of anti-venom; biomedical research; species identification; determination of geographic origin; sex determination; individual identification; parentage testing; toxicology analysis; disease testing/diagnosis, including serology; age analysis

  1. This corresponds to Annex XI to Regulation (EC) No 865/2006, the latest version of which should be checked for any recent amendments. ↩

  2. https://www.unodc.org/documents/Wildlife/Guidelines_Ivory.pdf ↩

  3. Republic of South Africa, Department of Environmental Affairs, Procedures for Rhino horn DNA Sampling. ↩

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